Philadelphia, May 29, 2020 - In a report in The Journal of Molecular Diagnostics, published by Elsevier, researchers describe a rapid, accurate novel assay for nondeletional alpha-thalassemia ...
High-resolution melting has proven to be a highly sensitive method for mutation discovery. PCR is performed in the presence of the double-strand DNA binding dye LCGreen® Plus. Samples are then ...
Study Shows How Melting Point Analysis Infers the Presence of and Identifies SNPs The melting temperature (T m) of a PCR product is dependent on both the length and sequence of the DNA. Melting curve ...
Multiple factors can affect these critical points; beside plastic ware, primer design, and master mix, the performance of the real-time PCR cycler itself influences the results. It is mandatory, for ...