Producing high yields of good-quality PCR products requires a complex combination of optimal chemistry, primer design, template quality, and cycling parameters. The details of these reaction ...

First, the BAC clone DNA is denatured into single stranded templates. Each of the three DOP-PCR primers used in the next step is flanked by known 5' and 3' anchor sequences with six random nucleotides ...

Optimizing the formulation of reagents for your qPCR assay requires careful experimental design that looks across several performance factors for each reagent formulation tested. These performance ...

Polymerase chain reaction, or PCR, was developed to identify a small fragment of a virus and create multiple copies, making detection easier. PCR is the gold standard in testing for COVID-19 and other ...